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ros fluorescence detection kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology ros fluorescence detection kit
    Ros Fluorescence Detection Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ros+fluorescence+assay+kit/Reactive+Oxygen+Species+(ROS)+Fluorometric+Assay+Kit/pm42492817-88-17-16
    Average 96 stars, based on 499 article reviews
    ros fluorescence detection kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts.
    Article Snippet: Objective: To investigate whether pyroptosis is induced by Porphyromonas gingivalis-lipopolysaccharide (P. gingivalis-LPS)/ adenosine triphosphate (ATP) through NF-κB/NLRP3/GSDMD signaling in human gingival fibroblasts (HGFs) and whether isoliquiritigenin (ISL) alleviates pyroptosis by inhibition of NF-κB/NLRP3/

    Article Title: ZhiXiaoSanZheng formula ameliorates podocyte injury in diabetic kidney disease by inhibiting ferroptosis: integrated network pharmacology and experimental validation
    Article Snippet: Creatinine (C011-2-1), urinary microalbumin (C035-2-1), blood urea nitrogen (C013-2-1), glutathione (A006-2-1), and malondialdehyde (A003-1-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. Iron concentration detection kits (E-BC-K773-M), Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M-48 T), and ROS Fluorescence Assay Kit (E-BC-K138-F) were purchased from Elabscience (Wuhan, China). .. RPMI 1640 medium (11,875,093/11879020, 1:1 dilution) was sourced from Gibco (New York, USA), fetal bovine serum (WS500T) from Austin (Shanghai, China), and penicillin/streptomycin (P1400) from Solarbio (Beijing, China).

    Article Title: ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis
    Article Snippet: .. 2'-7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) was used to quantify the ROS levels according to the protocol of the ROS fluorescence assay kit (E-BC-K138-F; Elabscience). .. GIST-T1 and GIST-T1 IR cells were plated in six-well plates and incubated for 24 h. After washing with PBS for three times, cells were labeled with 5 μM DCFH-DA under standard conditions for 30 min, cells were collected, and fluorescence intensity of DCF was detected using a microplate reader (SMR16.1, USCNK).

    Article Title: aPD-L1-facilitated theranostic and tumor microenvironment remodeling of pancreatic cancer via docetaxel-loaded phase-transformation nanoparticles triggered by low-intensity pulsed ultrasound.
    Article Snippet: .. The cells were subsequently cultured for an additional 24 h. The intracellular ROS levels were detected with an ROS fluorescence assay kit (#E-BC-K138-F, Elabscience), and the cell nuclei were labeled with Hoechst 33,342 (#62249, Thermo Scientific). ..

    Article Title: ZhiXiaoSanZheng formula ameliorates podocyte injury in diabetic kidney disease by inhibiting ferroptosis: integrated network pharmacology and experimental validation.
    Article Snippet: Creatinine (C011-2-1), urinary microalbumin (C0352-1), blood urea nitrogen (C013-2-1), glutathione (A006-2-1), and malondialdehyde (A003-1-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. Iron concentration detection kits (E-BC-K773-M), Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M-48 T), and ROS Fluorescence Assay Kit (E-BC-K138-F) were purchased from Elabscience (Wuhan, China). .. RPMI 1640 medium (11,875,093/11879020, 1:1 dilution) was sourced from Gibco (New York, USA), fetal bovine serum (WS500T) from Austin (Shanghai, China), and penicillin/streptomycin (P1400) from Solarbio (Beijing, China).

    Article Title: aPD-L1-facilitated theranostic and tumor microenvironment remodeling of pancreatic cancer via docetaxel-loaded phase-transformation nanoparticles triggered by low-intensity pulsed ultrasound
    Article Snippet: .. The cells were subsequently cultured for an additional 24 h. The intracellular ROS levels were detected with an ROS fluorescence assay kit (#E-BC-K138-F, Elabscience), and the cell nuclei were labeled with Hoechst 33,342 (#62249, Thermo Scientific). ..

    Article Title: Unraveling morphine tolerance: CCL2 induces spinal cord apoptosis via inhibition of Nrf2 signaling pathway and PGC-1α-mediated mitochondrial biogenesis.
    Article Snippet: .. The level of ROS was measured using the ROS Fluorescence Assay Kit (Elabscience Biotechnology Co., Ltd., Wuhan, China). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Isoliquiritigenin alleviates P. gingivalis-LPS/ATP-induced pyroptosis by inhibiting NF-κB/ NLRP3/GSDMD signals in human gingival fibroblasts.
    Article Snippet: Objective: To investigate whether pyroptosis is induced by Porphyromonas gingivalis-lipopolysaccharide (P. gingivalis-LPS)/ adenosine triphosphate (ATP) through NF-κB/NLRP3/GSDMD signaling in human gingival fibroblasts (HGFs) and whether isoliquiritigenin (ISL) alleviates pyroptosis by inhibition of NF-κB/NLRP3/

    Concentration Assay:

    Article Title: ZhiXiaoSanZheng formula ameliorates podocyte injury in diabetic kidney disease by inhibiting ferroptosis: integrated network pharmacology and experimental validation
    Article Snippet: Creatinine (C011-2-1), urinary microalbumin (C035-2-1), blood urea nitrogen (C013-2-1), glutathione (A006-2-1), and malondialdehyde (A003-1-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. Iron concentration detection kits (E-BC-K773-M), Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M-48 T), and ROS Fluorescence Assay Kit (E-BC-K138-F) were purchased from Elabscience (Wuhan, China). .. RPMI 1640 medium (11,875,093/11879020, 1:1 dilution) was sourced from Gibco (New York, USA), fetal bovine serum (WS500T) from Austin (Shanghai, China), and penicillin/streptomycin (P1400) from Solarbio (Beijing, China).

    Article Title: ZhiXiaoSanZheng formula ameliorates podocyte injury in diabetic kidney disease by inhibiting ferroptosis: integrated network pharmacology and experimental validation.
    Article Snippet: Creatinine (C011-2-1), urinary microalbumin (C0352-1), blood urea nitrogen (C013-2-1), glutathione (A006-2-1), and malondialdehyde (A003-1-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. Iron concentration detection kits (E-BC-K773-M), Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M-48 T), and ROS Fluorescence Assay Kit (E-BC-K138-F) were purchased from Elabscience (Wuhan, China). .. RPMI 1640 medium (11,875,093/11879020, 1:1 dilution) was sourced from Gibco (New York, USA), fetal bovine serum (WS500T) from Austin (Shanghai, China), and penicillin/streptomycin (P1400) from Solarbio (Beijing, China).

    Colorimetric Assay:

    Article Title: ZhiXiaoSanZheng formula ameliorates podocyte injury in diabetic kidney disease by inhibiting ferroptosis: integrated network pharmacology and experimental validation
    Article Snippet: Creatinine (C011-2-1), urinary microalbumin (C035-2-1), blood urea nitrogen (C013-2-1), glutathione (A006-2-1), and malondialdehyde (A003-1-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. Iron concentration detection kits (E-BC-K773-M), Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M-48 T), and ROS Fluorescence Assay Kit (E-BC-K138-F) were purchased from Elabscience (Wuhan, China). .. RPMI 1640 medium (11,875,093/11879020, 1:1 dilution) was sourced from Gibco (New York, USA), fetal bovine serum (WS500T) from Austin (Shanghai, China), and penicillin/streptomycin (P1400) from Solarbio (Beijing, China).

    Article Title: ZhiXiaoSanZheng formula ameliorates podocyte injury in diabetic kidney disease by inhibiting ferroptosis: integrated network pharmacology and experimental validation.
    Article Snippet: Creatinine (C011-2-1), urinary microalbumin (C0352-1), blood urea nitrogen (C013-2-1), glutathione (A006-2-1), and malondialdehyde (A003-1-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. Iron concentration detection kits (E-BC-K773-M), Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M-48 T), and ROS Fluorescence Assay Kit (E-BC-K138-F) were purchased from Elabscience (Wuhan, China). .. RPMI 1640 medium (11,875,093/11879020, 1:1 dilution) was sourced from Gibco (New York, USA), fetal bovine serum (WS500T) from Austin (Shanghai, China), and penicillin/streptomycin (P1400) from Solarbio (Beijing, China).

    Cell Culture:

    Article Title: aPD-L1-facilitated theranostic and tumor microenvironment remodeling of pancreatic cancer via docetaxel-loaded phase-transformation nanoparticles triggered by low-intensity pulsed ultrasound.
    Article Snippet: .. The cells were subsequently cultured for an additional 24 h. The intracellular ROS levels were detected with an ROS fluorescence assay kit (#E-BC-K138-F, Elabscience), and the cell nuclei were labeled with Hoechst 33,342 (#62249, Thermo Scientific). ..

    Article Title: aPD-L1-facilitated theranostic and tumor microenvironment remodeling of pancreatic cancer via docetaxel-loaded phase-transformation nanoparticles triggered by low-intensity pulsed ultrasound
    Article Snippet: .. The cells were subsequently cultured for an additional 24 h. The intracellular ROS levels were detected with an ROS fluorescence assay kit (#E-BC-K138-F, Elabscience), and the cell nuclei were labeled with Hoechst 33,342 (#62249, Thermo Scientific). ..

    Labeling:

    Article Title: aPD-L1-facilitated theranostic and tumor microenvironment remodeling of pancreatic cancer via docetaxel-loaded phase-transformation nanoparticles triggered by low-intensity pulsed ultrasound.
    Article Snippet: .. The cells were subsequently cultured for an additional 24 h. The intracellular ROS levels were detected with an ROS fluorescence assay kit (#E-BC-K138-F, Elabscience), and the cell nuclei were labeled with Hoechst 33,342 (#62249, Thermo Scientific). ..

    Article Title: aPD-L1-facilitated theranostic and tumor microenvironment remodeling of pancreatic cancer via docetaxel-loaded phase-transformation nanoparticles triggered by low-intensity pulsed ultrasound
    Article Snippet: .. The cells were subsequently cultured for an additional 24 h. The intracellular ROS levels were detected with an ROS fluorescence assay kit (#E-BC-K138-F, Elabscience), and the cell nuclei were labeled with Hoechst 33,342 (#62249, Thermo Scientific). ..



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    Image Search Results


    PEM induces ferroptosis in cells. A549 cells were grown with or without PEM (100 nM) for 72 h. (A) Heatmap summarizing RNA-seq data for the control and PEM-treated A549 cells with 744 upregulated genes (Red) and 412 downregulated genes (Green). (B) Volcano plot of differentially expressed genes between the control and PEM-treated cells as determined by RNA-seq. (C) KEGG enrichment analysis of PEM-regulated genes. The A549 or H1299 cells were subjected to PEM (100 nM), or DDP (200 nM), or PEM (100 nM) and DDP (200 nM) for 72 h. (D) The intracellular ROS was tested by flow cytometry, and the quantification of fluorescence intensities is shown. The levels of GSH (E) , SOD (F) , MDA (G) , and intercellular iron (H) were measured using appropriate kits, and the corresponding statistical results are shown. Western blot was used to detect (I) the expression of anti-ferroptosis proteins (SLC7A11, GPX4, DHODH, FSP1, and GCH1) and (J) FPN1, FTH1, FTL, and (K) the expression of pro-ferroptosis proteins (ACSL4, 12LOX, COX2, DMT1, TFR1, and TF) in A549 cells. ( * P < 0.01; ** P < 0.01; *** P < 0.005; **** P < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Pemetrexed sensitizes cisplatin therapy by inducing ferroptosis in NSCLC cells

    doi: 10.3389/fphar.2025.1764937

    Figure Lengend Snippet: PEM induces ferroptosis in cells. A549 cells were grown with or without PEM (100 nM) for 72 h. (A) Heatmap summarizing RNA-seq data for the control and PEM-treated A549 cells with 744 upregulated genes (Red) and 412 downregulated genes (Green). (B) Volcano plot of differentially expressed genes between the control and PEM-treated cells as determined by RNA-seq. (C) KEGG enrichment analysis of PEM-regulated genes. The A549 or H1299 cells were subjected to PEM (100 nM), or DDP (200 nM), or PEM (100 nM) and DDP (200 nM) for 72 h. (D) The intracellular ROS was tested by flow cytometry, and the quantification of fluorescence intensities is shown. The levels of GSH (E) , SOD (F) , MDA (G) , and intercellular iron (H) were measured using appropriate kits, and the corresponding statistical results are shown. Western blot was used to detect (I) the expression of anti-ferroptosis proteins (SLC7A11, GPX4, DHODH, FSP1, and GCH1) and (J) FPN1, FTH1, FTL, and (K) the expression of pro-ferroptosis proteins (ACSL4, 12LOX, COX2, DMT1, TFR1, and TF) in A549 cells. ( * P < 0.01; ** P < 0.01; *** P < 0.005; **** P < 0.001).

    Article Snippet: Intracellular ROS fluorescence assay kits (WLA131; Wanleibio, China) were used to measure the levels of ROS according to the kit instructions.

    Techniques: RNA Sequencing, Control, Flow Cytometry, Fluorescence, Western Blot, Expressing